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Abstract Title:

Lectin from red algae Amansia multifida Lamouroux: Extraction, characterization and anti-inflammatory activity.

Abstract Source:

Int J Biol Macromol. 2020 Dec 31 ;170:532-539. Epub 2020 Dec 31. PMID: 33388321

Abstract Author(s):

Jacilane Ximenes Mesquita, Tarcisio Vieira de Brito, Thais Pontes Carvalho Fontenelle, Renan Oliveira Silva Damasceno, Marcellus Henrique Loiola Ponte de Souza, José Luiz de Souza Lopes, Leila Maria Beltramini, André Luiz Dos Reis Barbosa, Ana Lúcia Ponte Freitas

Article Affiliation:

Jacilane Ximenes Mesquita

Abstract:

Seaweed lectins are very promising biotechnological tools that also gain prominence when applied to the pharmacology field. The purpose of the present work was to isolate and characterize lectin from the red algae Amansia multifida and subsequently test it in general inflammation models. The lectin was purified by ion exchange chromatography, characterized with two-dimensional electrophoresis, automated analysis of amino acid sequences and circular dichroism spectroscopy. The pharmacological tests performed were paw edema induced by carrageenan or rapid inflammatory mediators, peritonitis induced by carrageenan and myeloperoxidase leukocyte count assays, glutathione and cytokine concentration. Our results have identified a 30 KDa molecular weight protein that presents a major secondary structure arranged inβ-strand elements (~43%). A fragment of 20 amino acid residues was sequenced and presented low identity to the known classes of lectins from marine alga. This lectin was able to modulate inflammatory parameters such as paw edema, leukocyte migration, oxidative stress and proinflammatory cytokines.Thus, the lectin from the seaweed Amansia multifida has evident anti-inflammatory properties because it acts by reducing the formation of edema by modulating the effect of vascular mediators, migration of neutrophils, proinflammatory cytokines and oxidative stress control.

Study Type : In Vitro Study

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