Article Publish Status: FREE
Abstract Title:

The Effect ofPolysaccharides on Pyroptosis-Associated AmyloidβOligomers-Induced Adult Retinal Pigment Epithelium 19 Cell Damage.

Abstract Source:

Int J Mol Sci. 2020 Jun 30 ;21(13). Epub 2020 Jun 30. PMID: 32629957

Abstract Author(s):

Ming Yang, Kwok-Fai So, Amy Cheuk Yin Lo, Wai Ching Lam

Article Affiliation:

Ming Yang


Age-related macular degeneration (AMD) is a sight-threatening disease with limited treatment options. We investigated whether amyloidβ(Aβ) could cause pyroptosis and evaluated the effects ofpolysaccharides (LBP) on Aβoligomers-induced retinal pigment epithelium 19 (ARPE-19) damage, which is an in vitro AMD model. Aβoligomers verified by Western blot were added to ARPE-19 cells with or without 24 h LBP treatment. Aβoligomers significantly decreased ARPE-19 cell viability with obvious morphological changes under light microscopy. SEM revealed swollen cells with a bubbling appearance and ruptured cell membrane, which are morphological characteristics of pyroptosis. ELISA results showed increased expression of IL-1β and IL-18, which are the final products of pyroptosis. LBP administration for 24 h had no toxic effects on ARPE-19 cells and improved cell viability and morphology while disrupting Aβoligomerization in a dose-dependent manner. Furthermore, Aβoligomers up-regulated the cellular immunoreactivity of pyroptosis markers including NOD-like receptors protein 3 (NLRP3), caspase-1, and membrane N-terminal cleavage product of GSDMD (GSDMD-N), which could be reversed by LBP treatment. Taken together, this study showed that LBP effectively protects the Aβoligomers-induced pyroptotic ARPE-19 cell damages by its anti-Aβoligomerization properties and its anti-pyroptotic effects.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.