Macrophage-targeted berberine-loaded β-glucan nanoparticles enhance the treatment of ulcerative colitis. - GreenMedInfo Summary
Macrophage-Targeted Berberine-Loadedβ-Glucan Nanoparticles Enhance the Treatment of Ulcerative Colitis.
Int J Nanomedicine. 2022 ;17:5303-5314. Epub 2022 Nov 14. PMID: 36406639
Yuying Xu
AIM: This study focuses on constructing of an anti-inflammatory drug delivery system by encapsulation of berberine in theβ-glucan nanoparticles and evaluates its effect on treating ulcerative colitis.
METHODS: β-Glucan and the anti-inflammatory drug berberine (BER) are self-assembled into nanoparticles to construct a drug delivery system (GLC/BER). The interaction between the drug and the carrier was characterized by circular dichroism, ultraviolet-visible spectroscopy, and dynamic light scattering. The anti-inflammatory effect of the GLC/BER was evaluated through a lipopolysaccharide (LPS)-induced RAW264.7 macrophage inflammation model and a sodium sulfate (DSS)-induced C57BL/6 mouse ulcerative colitis model.
RESULTS: The GLC/BER nanoparticles have a particle size of 80-120 nm and a high encapsulation efficiency of 37.8±4.21%. In the LPS-induced RAW264.7 macrophage inflammation model, GLC/BER significantly promoted the uptake of BER by RAW264.7 cells. RT-PCR and ELISA assay showed that it could significantly inhibit the inflammatory factors including IL-1β, IL-6 and COX-2. Furthermore, GLC/BER shows inhibiting effect on the secretion of pro-inflammatory factors such as IL-1βand IL-6, down-regulating the production of nitrite oxide; in animal studies, GLC/BER was found to exert a relieving effect on mice colitis.
CONCLUSION: The study found that GLC/BER has an anti-inflammatory effect in vitro and in vivo, and the GLC carrier improves the potency and bioavailability of BER, providing a new type of nanomedicine for the treatment of colitis.