Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells.
Antioxidants (Basel). 2020 Sep 2 ;9(9). Epub 2020 Sep 2. PMID: 32887408
To demonstrate the anti-inflammatory activity offruit extract, human bronchial epithelial cells (BEAS-2B) were treated with lipopolysaccharide (LPS) and the effects of aronia bioactive fraction (ABF), anthocyanin enriched extract from the fruit ofwere evaluated. Following pretreatment with ABFat 10-25µg /mL, BEAS-2B cells were exposed to LPS and the expression of inflammatory mediators (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-8, regulated upon activation, normal T cell expressed and presumably secreted [RANTES], IL-1β, cyclooxygenase-2 [COX-2], and inducible nitric oxide synthase [iNOS]) was analyzed. In LPS-stimulated BEAS-2B cells, ABFpretreatment significantly decreased the mRNA expression of TNF-α, IL-6, IL-8, RANTES, IL-1β, and COX-2 at doses of 10 and 25 µg/mL. ABFalso attenuated the secretion of TNF-α, IL-6, IL-8, and RANTES protein, as demonstrated by enzyme linked immunosorbent assay. Western blot analyses revealed the decreased expression of COX-2 and iNOS following ABFtreatment. ROS production was decreased, and the cell cycle was arrested at the G0/G1 and S phases following ABFpretreatment. Our results suggest that ABFmay have potential as a nutraceutical agent for the suppression of airway inflammation.