Methanol soluble fraction of fruits ofpossesses significant antidiarrheal activities.
Heliyon. 2020 Jan ;6(1):e03112. Epub 2019 Dec 31. PMID: 31909276
Medicinal plants are the major sources of traditional treatment of disease in Indian subcontinent due to abundant presence of plants and vast side effects of synthetic drug. The present study was subjected to observethrombolytic, antibacterial, andantidiarrheal activities of methanol soluble fraction of fruits of. In thrombolytic activity assay, various concentrations (2─ 10 mg/ml) of methanol soluble fraction was used and dose dependently less potent activity was found. The maximum clot lysis 18.33% () was achieved at 10 mg/ml of methanolic fruit extract, whereas standard drug streptokinase showed 55.50% () clot lysis. In antibacterial assay, disc diffusion method was used comprising two gram positive () and two gram negative () bacteria. None of four (0.25, 0.5, 1, and 5 mg/disc) concentration of fruit extract showed antibacterial potentiality, whereas standard amikacin (3 mg/disc) revealed strong antibacterial activities (=~ 23─ 24 mm of MIC). To evaluate antidiarrheal activity, castor oil induced diarrhea was created in Swiss albino mice and different doses (100, 200, and 400 mg/kgbw) of fruit extract was introduced post orally. All of three different doses of fruit extract showed significant (p<0.05─ 0.001) antidiarrheal activities. Notably, the percent inhibition of diarrhea by methanolic extract of fruits ofwas found to be 58.38% at a dose of 400 mg/kgbw. The effect of vehicle saline (10 ml/kgbw) was considered as control and loperamide (5 mg/kgbw) as standard that provided 67.01% inhibition of diarrhea. The results suggest that, the fruits ofpossess potent antidiarrheal properties, providing scientific basis of using the plant parts in the treatment of diarrheal disease.