Neutralization ofvenom induced lethality, edema and myonecrosis by ethanolic root extract of.
Toxicol Rep. 2017 ;4:637-645. Epub 2017 Nov 24. PMID: 29234602
K S Rajesh
, commonly known as job's tear, is a tall grain-bearing tropical plant of the family. The ethanolic root extract (ERE) of the plant was investigated for the first time for anti-venom activity against Indian cobravenom.studies were conducted to determine neutralization of phospholipase A(PLA) activity of thevenom by the ERE. ERE showed significant inhibition of PLAactivity, which was further confirmed from effective neutralization of human red blood cells (HRBC) lysis induced by the venom. In addition, venom-induced proteolysis, fibrinogenolysis, DNase activity were also neutralized by the ERE, which contained carbohydrates, glycolides, resins and tannins. Oral administration of ERE at doses levels 100, 200 and 400 mg/kg effectively inhibitedvenom-induced lethality in mice. Myotoxicity induced byvenom, measured by creatine kinase activity in rats was significantly neutralized by the ERE at a dose of 200 mg/kg. Stigmasterol, as one of the component isolated from the ERE, was found to have venom phospholipase Ainhibition potential, which was confirmed by molecular docking studies with PLA. In summary, these studies indicate the ability of ERE ofto effectively neutralize the toxic effects of the venom is, in part, contributed by the inhibition of PLAactivity among other venom-derived factors.