Abstract Title:

Oxidation by thimerosal increases calcium levelsin renal tubular cells.

Abstract Source:

Pharmacol Toxicol. 2003 Sep ;93(3):123-7. PMID: 12969436

Abstract Author(s):

Chung-Ren Jan, Bang-Ping Jiann, Yih-Chau Lu, Hong-Tai Chang, Chia-Cheng Yu, Wei-Chung Chen, Jong-Khing Huang

Article Affiliation:

Chung-Ren Jan


The effect of thimerosal, a reactive oxidant, on cytoplasmic free Ca2+ concentrations ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells was explored by using the Ca2+-sensitive dye fura-2. Thimerosal acted in a concentration-dependent manner with an EC50 of 0.5 microM. The Ca2+ signal comprised a gradual rise and a sustained elevation. Removal of extracellular Ca2+ reduced 80% of the signal. In Ca2+-free medium, the [Ca2+]i rise induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) was completely inhibited by pretreatment with 5 microM thimerosal. The thimerosal (5 microM)-induced Ca2+ release was not changed by inhibition of phospholipase C with 2 microM U73122. Collectively, this study shows that thimerosal induced [Ca2+]i rises in renal tubular cells via releasing store Ca2+ from the endoplasmic reticulum Ca2+ stores in a manner independent of phospholipase C activity.

Study Type : In Vitro Study
Additional Links
Problem Substances : Thimerosal : CK(799) : AC(190)
Adverse Pharmacological Actions : Oxidant : CK(595) : AC(235)

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