p-coumaric acid ameliolates atopic dermatitis-like skin lesions. - GreenMedInfo Summary
-coumaric acid, an active ingredient of, ameliolates atopic dermatitis-like skin lesions through inhibition of thymic stromal lymphopoietin in mice.
J Ginseng Res. 2021 Jan ;45(1):176-182. Epub 2020 Jul 5. PMID: 33437169
Background: Atopic dermatitis (AD) is associated with chronic skin inflammatory reactions.-coumaric acid (CA) is an active ingredient ofMeyer (Araliaceae).
Methods: Here, we estimated an anti-AD effect ofCA on activated mast cells, activated splenocytes, and a mouse model of AD. Cytokines levels were measured by ELISA and protein activation was analyzed by Western blotting. 2,4-dinitrofluorobenzene (DNFB) was used to induce AD-like skin lesions.
Results: The treatment withCA suppressed the productions and mRNA expressions of thymic stromal lymphopoietin (TSLP), TNF-ɑ, IL-6, and IL-1β in HMC-1 cells.CA downregulated the expressions of RIP2 and caspase-1, phosphorylated-(p)p38/pJNK/pERK, and pIKKβ/pIkBɑ/NF-κB in HMC-1 cells.CA also decreased the productions of TSLP, TNF-ɑ, IL-6, IL-4, and IFN-γ in the supernatant of stimulated splenic cells. Comparing to DNFB-sensitized control group,CA-treated group alleviated pathological changes of AD-like lesions.CA decreased the proteins and mRNA expressions levels of TSLP, IL-6, and IL-4 in the skin lesions. Caspase-1 activation was also downregulated byCA treatment in the AD-like lesions. The serum levels of histamine, IgE, TSLP, TNF-ɑ, IL-6, and IL-4 were suppressed following treatment withCA.
Conclusion: This study suggests thatCA has the potential to improve AD by suppressing TSLP as well as inflammatory cytokines via blocking of caspase-1/NF-κB signal cascade.