[Role of PI3K/Akt pathway in effect of paeoniflorin against Aβ25-35-induced PC12 cell injury].
Zhongguo Zhong Yao Za Zhi. 2014 Oct ;39(20):4045-9. PMID: 25751960
OBJECTIVE: To study the role of PI3K/Akt pathway in the neuroprotective effect of paeoniflorin on PC12 cells.
METHOD: The paeoniflorin group (5, 10, 20μmol · L(-1)) was pretreated for 30 min, and then added with Aβ25-35 (20 μmol · L(-1)) for interaction for 24 h. Inhibitor LY294002 (10 μmol · L(-1)) was pretreated for 30 min before the action of paeoniflorin (10 μmol · L(-1)). The MTT colorimetric method was used to detect the cell viability. The apoptosis rate was tested by the FITC-Annexin V/PI staining. The protein expression of p-AKT, Bax, Bcl-2 and cleaved caspase-3 protein were detected by Western blot analysis.
RESULT: Paeoniflorin could significantly inhibit the Aβ25-35-induced PC12 cell toxicity and apoptosis. Its protection effect may be achieved by up- regulating AKT phosphorylation level, increasing Bcl-2 protein expression, reducing Bax protein expression, inhibiting the activation of caspase-3. Inhibitor LY294002 could weaken the above protective effects of paeoniflorin.
CONCLUSION: Paeoniflorin could activate PI3K/Akt signaling pathway to protect the PC12 cell injury induced by Aβ25-35.