Unraveling the Novel Protective Effect of Patchouli Alcohol Against-Induced Gastritis: Insights Into the Molecular Mechanismand.
Front Pharmacol. 2018 ;9:1347. Epub 2018 Nov 22. PMID: 30524287
Patchouli alcohol (PA), a natural tricyclic sesquiterpene extracted from(Blanco) Benth. (Labiatae), has been found to exhibit anti-and anti-inflammatory properties. In this study, we investigated the protective effect of PA againstinduced gastritisand, and determined the underlying mechanism. In theexperiment, a C57BL/6 mouse model of gastritis was established usingSS1, and treatments with standard triple therapy or 5, 10, and 20 mg/kg PA were performed for 2 weeks. Results indicated that PA effectively attenuated oxidative stress by decreasing contents of intracellular reactive oxygen species (ROS) and malonyldialdehyde (MDA), and increasing levels of non-protein sulfhydryl (NP-SH), catalase and glutathione (GSH)/glutathione disulphide (GSSG). Additionally, treatment with PA significantly attenuated the secretions of interleukin 1 beta (IL-1β), keratinocyte chemoattractant and interleukin 6 (IL-6). PA (20 mg/kg) significantly protected the gastric mucosa from-induced damage. In theexperiment, GES-1 cells were cocultured withNCTC11637 at MOI = 100:1 and treated with different doses of PA (5, 10, and 20μg/ml). Results indicated that PA not only significantly increased the cell viability and decreased cellular lactate dehydrogenase (LDH) leakage, but also markedly elevated the mitochondrial membrane potential and remarkably attenuated GES-1 cellular apoptosis, thereby protecting gastric epithelialcells against injuries caused by. PA also inhibited the secretions of pro-inflammatory factors, such as monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-α (TNF-α) and IL-6. Furthermore, after PA treatment, the combination of NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) and cysteine-aspartic proteases 1 (CASPASE-1), the expression levels of NLRP3 inflammasome-related proteins, such as thioredoxin-interacting protein (TXNIP), pro-CASPASE-1, cle-CASPASE-1, and NLRP3 and genes (and) were significantly decreased as compared to the model group. In conclusion, treatment with PA for 2 weeks exhibited highly efficient protective effect against-induced gastritis and related damages. The underlying mechanism might involve antioxidant activity, inhibition of pro-inflammatory factor and regulation of NLRP3 inflammasome function. PA exerted anti-and anti-gastritis effects and thus had the potential to be a promising candidate for treatment of-related diseases.