PD-1-expressing SARS-CoV-2-specific CD8+ T cells are not exhausted, but functional in patients with COVID-19. - GreenMedInfo Summary
PD-1-Expressing SARS-CoV-2-Specific CD8T Cells Are Not Exhausted, but Functional in Patients with COVID-19.
Immunity. 2021 01 12 ;54(1):44-52.e3. Epub 2020 Dec 14. PMID: 33338412
Min-Seok Rha
Memory T cell responses have been demonstrated in COVID-19 convalescents, but ex vivo phenotypes of SARS-CoV-2-specific T cells have been unclear. We detected SARS-CoV-2-specific CD8T cells by MHC class I multimer staining and examined their phenotypes and functions in acute and convalescent COVID-19. Multimercells exhibited early differentiated effector-memory phenotypes in the early convalescent phase. The frequency of stem-like memory cells was increased among multimercells in the late convalescent phase. Cytokine secretion assays combined with MHC class I multimer staining revealed that the proportion of interferon-γ (IFN-γ)-producing cells was significantly lower among SARS-CoV-2-specific CD8T cells than those specific to influenza A virus. Importantly, the proportion of IFN-γ-producing cells was higher in PD-1cells than PD-1cells among multimercells, indicating that PD-1-expressing, SARS-CoV-2-specific CD8T cells are not exhausted, but functional. Our current findings provide information for understanding of SARS-CoV-2-specific CD8T cells elicited by infection or vaccination.