Abstract Title:

REC-2006--A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation-induced Damage by Reducing the Initial Damage and Enhancing Its Repair in vivo.

Abstract Source:

Evid Based Complement Alternat Med. 2009 Dec 11. PMID: 20008078

Abstract Author(s):

Pankaj Chaudhary, Sandeep Kumar Shukla, Rakesh Kumar Sharma

Article Affiliation:

Brookhaven National Laboratory, Upton, NY 11973, USA. [email protected]; [email protected]


Podophyllum hexandrum, a perennial herb commonly known as the Himalayan May Apple, is well known in Indian and Chinese traditional systems of medicine. P. hexandrum has been widely used for the treatment of venereal warts, skin infections, bacterial and viral infections, and different cancers of the brain, lung and bladder. This study aimed at elucidating the effect of REC-2006, a bioactive fractionated extract from the rhizome of P. hexandrum, on the kinetics of induction and repair of radiation-induced DNA damage in murine thymocytes in vivo. We evaluated its effect on non-specific radiation-induced DNA damage by the alkaline halo assay in terms of relative nuclear spreading factor (RNSF) and gene-specific radiation-induced DNA damage via semi-quantitative polymerase chain reaction. Whole body exposure of animals with gamma rays (10 Gy) caused a significant amount of DNA damage in thymocytes (RNSF values 17.7 +/- 0.47, 12.96 +/- 1.64 and 3.3 +/- 0.014) and a reduction in the amplification of beta-globin gene to 0, 28 and 43% at 0, 15 and 60 min, respectively. Administrating REC-2006 at a radioprotective concentration (15 mg kg(-1) body weight) 1 h before irradiation resulted in time-dependent reduction of DNA damage evident as a decrease in RNSF values 6.156 +/- 0.576, 1.647 +/- 0.534 and 0.496 +/- 0.012, and an increase in beta-globin gene amplification 36, 95 and 99%, at 0, 15 and 60 min, respectively. REC-2006 scavenged radiation-induced hydroxyl radicals in a dose-dependent manner stabilized DPPH free radicals and also inhibited superoxide anions. Various polyphenols and flavonoides present in REC-2006 might contribute to scavenging of radiation-induced free radicals, thereby preventing DNA damage and stimulating its repair.

Study Type : Animal Study

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