Anti-inflammatory potential of black carrot (Daucus carota L.) polyphenols in a co-culture model of intestinal Caco-2 and endothelial EA.hy926 cells.
Mol Nutr Food Res. 2016 Aug 26. Epub 2016 Aug 26. PMID: 27561918
SCOPE: The present study was developed to determine the ability of polyphenol-rich black carrot and its by-products, i.e., peel and pomace, to modulate the inflammatory response in tumor necrosis factor-α (TNF-α) treated endothelial cells after gastrointestinal digestion and in a co-culture of intestinal Caco-2 and endothelial EA.hy926 cell model.
RESULTS: The results indicated that after 4 h of treatment, the transport of anthocyanins and phenolic acids was higher for digested samples (1.3-7%) compared to undigested ones (0-3.3%). The transported polyphenols were able to down-regulate the secretion of pro-inflammatory markers, i.e., interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1), under normal and TNF-α induced inflammatory conditions. The most pronounced protective effects were observed with digested samples under inflammatory conditions, which significantly decreased the secretion of all markers from 120-203% down to 34-144% (p<0.001).
CONCLUSIONS: Overall, these results show that the polyphenol-rich black carrot absorption products may function through an inhibitory regulation of the inflammatory cascade in endothelial cells. This article is protected by copyright. All rights reserved.