Potential Application ofL. andL. Leaf Essential Oils as Antioxidant and of Cholinesterases Inhibitors.
Antioxidants (Basel). 2018 Dec 21 ;8(1). Epub 2018 Dec 21. PMID: 30577585
The aim of this work is to investigate the in vitro acetylcholinesterase (AChE) and butyrycholinesterase (BChE) inhibitory activities of essential oils obtained by hydrodistillation from the leaves ofandin relation to their composition, analysed by Gas Chromatography⁻Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses, at different times. Moreover, considering the role of free radicals in the progression of neurodegenerative disorders, the antioxidant properties of essential oils were investigated by using, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and β-carotene bleaching tests. The relative antioxidant capacity index (RACI) was used to achieve more comprehensive comparison between analysed antioxidant effects of essential oils.oils were more active thanoils against AChE. Against BChE, the most active was the essential oil fromleaves collected in August with an ICvalue of 95.80μg/mL. This oil exerted the highest inhibitory activity of lipid peroxidation with ICvalues of 11.15 and 11.39μg/mL after 30 and 60 min of incubation, respectively. All samples demonstrated a remarkable ABTS radicals scavenging activity, with ICvalues in the range 0.45⁻0.57 μg/mL in comparison to the positive control, ascorbic acid.