Abstract Title:

Preventive effects of astragaloside IV and its active sapogenin cycloastragenol on cardiac fibrosis of mice by inhibiting the NLRP3 inflammasome.

Abstract Source:

Eur J Pharmacol. 2018 Aug 15 ;833:545-554. Epub 2018 Jun 18. PMID: 29913124

Abstract Author(s):

Yu Wan, Lu Xu, Yunxia Wang, Nuerbiya Tuerdi, Min Ye, Rong Qi

Article Affiliation:

Yu Wan


Cardiac fibrosis is a common feature of many cardiac pathophysiologic conditions. Recently, it has been shown that the activation of NLRP3 inflammasome plays an important role in the pathophysiology of cardiac fibrosis. Here, the inhibitory effects and possible mechanism of astragaloside IV (AST) and its active sapogenin cycloastragenol (CAG) on isoproterenol (ISO)-induced cardiac fibrosis were investigated. In our study, BALB/c mice were subcutaneously injected with 5 mg/kg ISO for 7 consecutive days to induce cardiac fibrosis. AST or CAG was administrated to the mice intragastrically at different doses beginning on the same day of ISO injection. Primary cardiac fibroblasts were isolated from the hearts of neonatal rats, and treated with 10 μmol/L ISO for 24 h with or without incubation of CAG simultaneously. The results indicated that 62.5 mg/kg CAG could significantly inhibit ISO-induced cardiac fibrosis, which was evidenced by sirius red staining, collagen volume fraction and mRNA expressions of collagen-1, collagen-3 and TGF-β1. Hematoxylin-eosin staining showed that 62.5 mg/kg CAG markedly reduced the inflammatory cell infiltration in heart tissues. To elucidate the related mechanism, NLRP3/caspase-1/IL-18 pathway was studied. The mRNA expressions of NLRP3, caspase-1, IL-18 and IL-6 in mice heart tissues were significantly down-regulated by 62.5 mg/kg CAG and 200 mg/kg AST. And incubation with 31.25 μg/ml CAG markedly attenuated ISO-induced mRNA over-expressions of NLRP3, caspase-1, IL-18 and IL-6 in primary cardiac fibroblasts. These findings showed that CAG effectively inhibited ISO-induced cardiac fibrosis, and both CAG and AST exhibited anti-fibrosis effects through inhibition of the NLRP3 inflammasome pathway.

Study Type : Animal Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.