Abstract Title:

Protective effects of anthocyanins from bilberry extract in rats exposed to nephrotoxic effects of carbon tetrachloride.

Abstract Source:

Chem Biol Interact. 2019 Feb 27 ;304:61-72. Epub 2019 Feb 27. PMID: 30825423

Abstract Author(s):

Dejan Popović, Gordana Kocić, Vuka Katić, Zorica Jović, Aleksandra Zarubica, Ljubinka Janković Veličković, Valentina Nikolić, Andrija Jović, Braca Kundalić, Violeta Rakić, Nataša Poklar Ulrih, Mihaela Skrt, Danka Sokolović, Ljubomir Dinić, Marko Stojanović, Aleksandar Milosavljević, Filip Veličković, Dušan Sokolović

Article Affiliation:

Dejan Popović


This study examined the nephroprotective effects of 15 different anthocyanins from the bilberry extract on the acute kidney injury caused by CCl. The acute nephrotoxicity in rats was induced 24 h after the treatment with a single dose of CCl(3 mL/kg, i.p.).The nephroprotective effects of the anthocyanins were examined in the animals that had been given the bilberry extract in a single dose of 200 mg of anthocyanins/kg daily, 7 days orally, while on the seventh day, 3 h after the last dose of anthocyanins, the animals received a single dose of CCl(3 mL/kg, i.p.) and were sacrificed 24 h later. When the nephrotoxicant alone was administered, it resulted in a substantial increase of the pro-oxidative (TBARS, CD, HO, XO, and GSSG) and pro-inflammatory markers (TNF-α, NO, and MPO), as well as a noticeable reduction of the antioxidant enzymes (CAT, SOD, POD, GPx, GST, GR) and GSH when compared to the results of the control group. Moreover, the application of CClsignificantly influenced a reduction of the renal function, as well as an increase in the sensitive and specific injury indicators of the kidney epithelial cells (β-microglobulin, NGAL, KIM1/TIM1) in the serum and urine of rats. The pretreatment of the animals poisoned with CClwith the anthocyanins from the bilberry extract led to a noticeable reduction in the pro-oxidative and pro-inflammatory markers with reduced consumption of the antioxidant defence kidney capacity, compared to the animals exposed to CClalone. Anthocyanins have been protective for the kidney parenchyma, with an apparent absence of the tubular and periglomerular necrosis, severe degenerative changes, inflammatory mononuclear infiltrates and dilatation of proximal and distal tubules, in contrast to the CCl-intoxicated animals. The nephroprotective effects of anthocyanins can be explained by strong antioxidant and anti-inflammatory effects achieved through the stabilization and neutralization of highly reactive and unstable toxic CClmetabolites.

Study Type : Animal Study

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