Protective Effects of Crocetin against Radiation-Induced Injury in Intestinal Epithelial Cells.
Biomed Res Int. 2020 ;2020:2906053. Epub 2020 Sep 8. PMID: 32964024
Background and Aims: Treatment options for radiation-induced intestinal injury (RIII) are limited. Crocetin has been demonstrated to exert antioxidant, antiapoptotic, and anti-inflammatory effects on various diseases. Here, we investigate the effects of crocetin on RIII.. IEC-6 cells exposed to 10 Gy of radiation were treated with different doses of crocetin (0, 0.1, 1, 10, and 100 M), and cell viability was assessed by CCK-8. The levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA), myeloperoxidase (MPO), tumor necrosis factor-(TNF-), interleukin-1(IL-1), and interferon-(IFN-) in culture supernatants were measured using colorimetric and ELISA kits, respectively. Cellular apoptosis was evaluated by Annexin V/PI double staining.
Results: Crocetin dose-dependently improved the survival of irradiated IEC-6 cells with the optimal dose of 10 M, as indicated by the reduction of cellular apoptosis, decreased levels of MDA, MPO, and proinflammatory cytokines (TNF-, IL-1, and IFN-), and increased activities of antioxidative enzymes (SOD, CAT, and GPx).
Conclusion: Our findings demonstrated that crocetin alleviated radiation-induced injury in intestinal epithelial cells, offering a promising agent for radioprotection.