Article Publish Status: FREE
Abstract Title:

Protective Effects of Silymarin and Silibinin against DNA Damage in Human Blood Cells.

Abstract Source:

Biomed Res Int. 2018 ;2018:6056948. Epub 2018 Oct 2. PMID: 30370304

Abstract Author(s):

Flávio Fernandes Veloso Borges, Carolina Ribeiro E Silva, Wanessa Moreira Goes, Fernanda Ribeiro Godoy, Fernanda Craveiro Franco, Jefferson Hollanda Véras, Elisa Flávia Luiz Cardoso Bailão, Daniela de Melo E Silva, Clever Gomes Cardoso, Aparecido Divino da Cruz, Lee Chen-Chen

Article Affiliation:

Flávio Fernandes Veloso Borges


Silymarin (SM), a standardized extract derived from(L.) Gaertn, is primarily composed of flavonolignans, with silibinin (SB) as its major active constituent. The present study aimed to evaluate the antigenotoxic activities of SM and SB using the alkaline comet assay in whole blood cells and to assess their effects on the expression of genes associated with carcinogenesis and chemopreventive processes. Different concentrations of SM or SB (1.0, 2.5, 5.0, and 7.5 mg/ml) were used in combination with the DNA damage-inducing agent methyl methanesulfonate (MMS, 800M) to evaluate their genoprotective potential. To investigate the role of SM and SB in modulating gene expression, we performed quantitative real-time PCR (qRT-PCR) analysis of five genes that are known to be involved in DNA damage, carcinogenesis, and/or chemopreventive mechanisms. Treatment with SM or SB was found to significantly reduce the genotoxicity of MMS, upregulate the expression ofand, and downregulate the expression ofand. We observed no significant changes inexpression levels following treatment with SM or SB. In conclusion, both SM and SB exerted antigenotoxic activities and modulated the expression of genes related to cell protection against DNA damage.

Study Type : In Vitro Study

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