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Abstract Title:

Puerarin Inhibits the Progression of Bladder Cancer by Regulating//Axis.

Abstract Source:

Cancer Biother Radiopharm. 2020 Oct 5. Epub 2020 Oct 5. PMID: 33016781

Abstract Author(s):

Lijun Du, Li Zhang, Fei Sun

Article Affiliation:

Lijun Du

Abstract:

Previous studies have shown puerarin to be a potential therapeutic drug for treatment of bladder cancer. But the role and possible molecular mechanism of puerarin remain unknown.Cell viability, apoptosis, migration, and invasion were assessed by Cell Counting Kit-8 (CCK-8), flow cytometry, and transwell assays, respectively. Western blot was used to measure the levels of all protein. Glucose consumption and lactate production were detected using a glucose and lactate assay kit. Circular RNA_0020394 (), microRNA-328-3p (), and nuclear receptor binding protein 1 () levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). The interaction between miRNA and circRNA or mRNA was confirmed using dual-luciferase reporter assay.experiments were performed to examine the effect of puerarin on tumor growth.Puerarin suppressed cell viability, migration, invasion, and glycolysis, and induced apoptosis in bladder cancer.was downregulated in puerarin-treated bladder cancer cells, andoverexpression attenuated the inhibitory effect of puerarin on cell progression. Moreover,could bind to, anddirectly targeted. Functionally,could reverse the promotion effect ofoverexpression on the progression of puerarin-treated cells, and silencingcounteracted the effects of anti-on puerarin-treated cells. Mechanically,could increaseexpression by acting assponge in puerarin-treated bladder cancer cells. Besides, puerarin inhibited tumorigenesisby increasingand decreasing the levels ofand.Puerarin impedes cell viability, migration, invasion, and glycolysis, and promoted apoptosis in bladder cancer by regulating//axis.

Study Type : In Vitro Study

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