n/a
Abstract Title:

Puerarin protects against HO-induced apoptosis of HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/Akt axis.

Abstract Source:

Placenta. 2022 Jul 6 ;126:202-208. Epub 2022 Jul 6. PMID: 35870270

Abstract Author(s):

Lidan He, Xiuyan Wu, Xia Zhang, Xuemei Li, Xin Lin, Yameng Huang, Jianbo Wu

Article Affiliation:

Lidan He

Abstract:

INTRODUCTION: Preeclampsia seriously affects the health of pregnant women and fetuses. It has been reported that puerarin has a positive therapeutic effect on the treatment of preeclampsia. In this study, oxidative stress-induced trophoblast cell injury was established to explore the potential interaction between puerarin and preeclampsia.

METHODS: A CCK-8 assay was performed to investigate the effect of puerarin on the viability of HTR-8/SVneo cells. To mimic oxidative stress-induced trophoblast cell injury, human villous trophoblasts (HTR-8/SVneo) were treated with HO. Then, the relationships among MMP2, VEGFA and miR-20a-5p in HTR-8/SVneo cells were confirmed using a dual-luciferase reporter assay. Finally, Western blot assays were performed to measure the expression levels of MMP2, VEGFA, p-Akt, Akt, Bcl-2 and cleaved caspase 3.

RESULTS: In this study, puerarin eliminated HO-induced cytotoxicity of HTR-8/SVneo cells. In addition, puerarin was able to reverse HO-induced apoptosis and metastasis inhibition in cells. Meanwhile, puerarin significantly abrogated HO-induced mitochondrial membrane potential (MMP) decline in HTR-8/SVneo cells. And, MMP2 and VEGFA were identified as direct targets of miR-20a-5p. Furthermore, puerarin reversed HO-induced growth inhibition in HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/Akt axis.

DISCUSSION: All these data indicated that puerarin could abolish HO-induced growth inhibition in HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/AKT axis.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.