n/a
Abstract Title:

[Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats].

Abstract Source:

Yao Xue Xue Bao. 2004 Jul ;39(7):481-5. PMID: 15493832

Abstract Author(s):

Hui-Jun Duan, Shu-Xia Liu, Yu-Jun Zhang, Qing-Juan Liu, Ning He, Ying-Min Li

Article Affiliation:

Hui-Jun Duan

Abstract:

AIM: To investigate the effect of puerarin on expressions of MMP-2 and TIMP-2 in the kidney of diabetic rats.

METHODS: Uninephrectomized male Wistar rats were used to induce diabetes by intraperitoneal injection of streptozocin (65 mg x kg(-1)). Puerarin was given daily by intraperitoneal injection from the third day of induction of diabetes for 16 weeks. Using in situ hybridization and immunohistochemistry to detect MMP-2, TIMP-2 mRNA expressions and MMP-2, TIMP-2, collagen IV and Laminin expressions in diabetic kidneys with image analysis system, Flow cytometry was used to detect the expressions of TGFbeta1, MMP-2 and TIMP-2.

RESULTS: Compared with those in kidneys of control group, expressions of MMP-2 mRNA and proteins were lower, while the expressions of both TGFbeta1 and TIMP-2 were higher in the diabetic kidney (P<0.05). The level of MMP-2 expression was advanced, while expression of TIMP-2 was reduced by puerarin treatment (P<0.05).

CONCLUSION: Puerarin showed some renal protective effect on diabetic nephropathy, partly through inhibition of excessive deposition of glomeruli extracellular matrix by up-regulating MMP-2 and down-regulating TIMP-2 expressions besides reducing the blood glucose.

Study Type : Animal Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.