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Article Publish Status: FREE
Abstract Title:

Resveratrol inhibits ACHN cells via regulation of histone acetylation.

Abstract Source:

Pharm Biol. 2020 Dec ;58(1):231-238. PMID: 32202448

Abstract Author(s):

Lili Dai, Lingyan Chen, Wenjing Wang, Peizheng Lin

Article Affiliation:

Lili Dai

Abstract:

The relationship between resveratrol and histone acetylation in renal cell carcinoma (RCC) has not yet been reported.To explore the functional role of resveratrol in RCC.Functional experiments were performed to determine proliferatio n of ACHN cells with treatment of resveratrol (0, 7.8125, 15.625, 31.25 and 62.5 μg/mL, for 12, 24 and 48 h of culture) or 0.1 μM SAHA. The enzyme activities of MMP-2/-9 were measured by gelatine zymography and histone acetylation by Western blot.When the cells were treated with 15.625, 31.25 and 62.5 μg/mL resveratrol, ACHN cells viability was 73.2 ± 3.5%, 61.4 ± 3.1%, 50.2 ± 4.7% for 12 h, 62.7 ± 4.5%, 52.4 ± 5.5%, 40.2 ± 3.8% for 24 h, and 60.8 ± 3.7%, 39.4 ± 5.1%, 37.6 ± 2.7% for 48 h, and the wound closure (%) of migration was increasedfrom 0.6 to 0.7, 0.85, 0.9 for 12 h and from 0.23 to 0.3, 0.48, 0.59 for 24 h. The invasion rate was 8.5 ± 0.9%, 7.4 ± 0.3% and 5.8 ± 0.6%, and cell cycle was arrested at G1 from 42.5 ± 2.9% to 55.3 ± 5.7%, 59.8 ± 3.4%, 68.7 ± 4.6%. MMP-2/-9 expression ( < 0.05) was inhibited by resveratrol. The protein levels of histone acetylation ( < 0.01) was increased by resveratrol.Our results suggest that these effects might be related to a high level of histone acetylation, and resveratrol can be considered as an alternative treatment for RCC.

Study Type : In Vitro Study

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