Effects of resveratrol, a grape polyphenol, on uterine contraction and Ca²+ mobilization in rats in vivo and in vitro.
Mutat Res. 1993 Nov;303(3):109-12. PMID: 21363935
Department of Nutrition and Health Sciences (S.-M.H.), School of Healthcare Management, Kainan University, Taoyuan 33857, Taiwan, Republic of China; Department of Physiology (K.-L.W., P.S.W.),
Dysmenorrhea is directly related to elevate prostaglandin F (PGF)(2α) levels. In Western medicine, this condition is treated using nonsteroidal antiinflammatory drugs. Because nonsteroidal antiinflammatory drugs produce many side effects, Chinese medicinal therapy is considered as a feasible alternative for treating dysmenorrhea. Many special physiological components used in Chinese medicine, such as resveratrol, have been isolated and identified. Resveratrol has many physiological functions, such as antioxidation and anticarcinogenic effects. However, the relationship between uterine smooth muscle contraction and resveratrol remains unknown. Here, we studied the in vitro and in vivo effects of resveratrol on uterine smooth muscle contraction. The uterus was separated from a female Sprague Dawley rat, and uterine smooth muscle contraction activity was measured and recorded. The results demonstrated that 1) resveratrol treatment inhibited PGF(2α)-, oxytocin-, acetylcholine-, and carbachol-induced uterine contractions in rats; 2) resveratrol inhibited uterine contractions stimulated by the Ca(2+) channel activator (Bay K 8644) and depolarization in response to high K(+) (KCl); 3) resveratrol inhibited PGF(2α)-induced increases in the [Ca(2+)]i inhuman uterine smooth muscle cells; 4) resveratrol could mimic Ca(2+) channel blockers to block Ca(2+) influx through voltage-operated Ca(2+) channels in the plasma membrane; and 5) resveratrol inhibited PGF(2α)-induced uterine contractions in rats in vivo. Resveratrol inhibited uterine contractions induced by PGF(2α) and high K(+) in a concentration-dependent manner in vitro; furthermore, it inhibited Ca(2+)-dependent uterine contractions. Thus, resveratrol consistently suppressed the increases in intracellular Ca(2+) concentrations ([Ca(2+)]i) induced by PGF(2α) and high K(+) concentrations. It can be assumed that resveratrol probably inhibited uterine contraction by blocking external Ca(2+) influx, leading to decreased [Ca(2+)]i. Therefore, resveratrol can be considered as a feasible alternative therapeutic agent for dysmenorrhea.