Abstract Title:

Effects of resveratrol on proliferation and apoptosis in rat ovarian theca-interstitial cells.

Abstract Source:

Mol Hum Reprod. 2010 Jan 12. Epub 2010 Jan 12. PMID: 20067985

Abstract Author(s):

Donna H Wong, Jesus A Villanueva, Amanda B Cress, Antoni J Duleba

Abstract:

Polycystic ovary syndrome (PCOS) is characterized by ovarian dysfunction and associated with ovarian theca-interstitial cell hyperplasia, hyperinsulinemia, systemic inflammation, and oxidative stress. This in vitro study tested whether rat theca-interstitial cell growth with or without insulin can be altered by resveratrol, a natural polyphenol with anti-carcinogenic, anti-inflammatory, anti-proliferative and anti-oxidant properties. Rat theca-interstitial cells were cultured with and without resveratrol and/or insulin, and the effects on DNA synthesis, number of viable cells and markers of apoptosis were evaluated. Resveratrol alone induced a potent concentration-dependent inhibition of cell growth by inhibiting DNA synthesis, decreasing the number of viable cells, and increasing the activity of executioner caspases 3 and 7; these effects of resveratrol counteracted the pro-proliferative and anti-apoptotic effects of insulin. Immunofluorescence analysis of cells incubated with resveratrol showed concentration-and time-dependent morphological changes consistent with apoptosis. The present findings indicate that resveratrol promotes apoptosis to reduce rat theca-interstitial cell growth in vitro as well as inhibiting insulin-induced rat theca-interstitial cell growth. This suggests a possibility that resveratrol and/or mechanisms mediating its effect may be relevant to the development of novel treatments for PCOS, which is characterized by both excessive ovarian mesenchyma growth and hyperinsulinemia.

Study Type : In Vitro Study

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