Abstract Title:

Investigation of porcine circovirus contamination in human vaccines.

Abstract Source:

Biologicals. 2012 Mar 6. Epub 2012 Mar 6. PMID: 22402185

Abstract Author(s):

Sarah M Gilliland, Lindsay Forrest, Heather Carre, Adrian Jenkins, Neil Berry, Javier Martin, Philip Minor, Silke Schepelmann

Article Affiliation:

Division of Virology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, United Kingdom.

Abstract:

DNA from porcine circovirus type 1 (PCV1) and 2 (PCV2) has recently been detected in two vaccines against rotaviral gastroenteritis from manufacturers A and B. We investigated if PCV1 sequences are present in other viral vaccines. We screened seeds, bulks and final vaccine preparations from ten manufacturers using qRT-PCR. We detected 3.8×10(3) to 1.9×10(7) PCV1 DNA copies/milliliter in live poliovirus seeds for inactivated polio vaccine (IPV) from manufacturer A, however, following inactivation and purification, the finished IPV was PCV1-negative. PCV1 DNA was not detectable in live polio preparations from other vaccine producers. There was no detectable PCV1 DNA in the measles, mumps, rubella and influenza vaccines analysed including material supplied by manufacturer A. We confirmed that the PCV1 genome in the rotavirus vaccine from manufacturer A is near full-length. It contains two mutations in the PCV cap gene, which may result from viral adaptation to Vero cells. Bulks of this vaccine contained 9.8×10(10) to 1.8×10(11) PCV1 DNA copies/millilitre and between 4.1×10(7) and 5.5×10(8) DNA copies were in the final doses. We found traces of PCV1 and PCV2 DNA in the rotavirus vaccine from manufacturer B. This highlights the issue of vaccine contamination and may impact on vaccine quality control.

Study Type : Review

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