Royal jelly protects male mice against nicotine-induced reproductive failure. - GreenMedInfo Summary

This study evaluated the possible protective effect of royal jelly (RJ) on sperm parameters and sperm malondialdehyde (MDA) concentration andfertilizing potential in nicotine (NIC) exposed male mice. Thrtiy-six male BALB/c mice were randomly divided into six groups (n = 6). Group 1 received normal saline, group 2 received 100 mg kgper day RJ, groups 3 and 4 received NIC at doses of 0.50 and 1.00 mg kgper day, respectively and groups 5 and 6 received NIC at doses of 0.50 and 1.00 mg kgper day, respectively plus RJ. Caudal epididymal sperm characteristics, lipid peroxidation andfertilizing capacity and embryo development were evaluated after 35 days. The NIC treatment caused a significant decrease in sperm motility and viability and fertilization rate along with poor blastocyst formation and increased sperm DNA damage and MDA levels. Moreover, the incidences of chromatin abnormality in spermatozoa were significantly higher in NIC-exposed mice than those of control. Nevertheless, RJ treatment improved sperm parameters andfertilization outcome as well as sperm lipid peroxidation level. Data from the current study suggest that RJ has a potential repro-protective action against NIC-induced sperm abnormalities and embryotoxicity in mice.