n/a
Article Publish Status: FREE
Abstract Title:

Screening active components fromfor pancreaticβ-cells protection.

Abstract Source:

Pharm Biol. 2020 Dec ;58(1):674-685. PMID: 32659127

Abstract Author(s):

Min Sun, Tiantian Zhu, Jinzhi Tong, Rezeng Caidan, Kaijin Wang, Guiqing Kai, Wenna Zhang, Lei Ru, Jiumei Pengcuo, Li Tong

Article Affiliation:

Min Sun

Abstract:

CONTEXT: species (Rosaceae) have been used in folk medicine to treat diabetes due to their hypoglycaemic activity.

OBJECTIVE: To screen the active components that act as hypoglycaemic agents inFocke and the underlying mechanisms.

MATERIALS AND METHODS: Aqueous stem extract ofwas incubated with MIN6β-cells, PBS was used as the blank control. Then the cells were washed, cell membrane-bound components were dissociated and identified by UPLC/MS. Total procyanidins (PCs) inwas enriched and the cytotoxicity and anti-proliferation onβ-cell were evaluated by MTT assay. PCs at 25, 50, and 75 μg/mL was applied for 24 h to determine its effects on palmitate (PA)-induced apoptosis and GSIS. Western blotting was employed to detect the protein expression of PI3K/Akt/FoxO1 signalling. The antioxidant indices were also measured.

RESULTS: β-Cell membrane-bound components were identified as three procyanidin B dimers and a C trimer. PCs showed no significant cytotoxicity up to a concentrations of 100 μg/mL. PCs treatment reversed the elevated apoptosis rate and impaired GSIS induced by PA. PCs markedly decreased the intracellularROS and MDA production and increased the SOD activity. Moreover, PCs promoted the phosphorylation of Akt and FoxO1, and regulated Pdx-1 and Bax expression in MIN6 cells.The active components that act as hypoglycaemic agents inare procyanidins, which protected MIN6 cells against PA-induced apoptosis by activating PI3K/Akt/FoxO1 signalling. These results indicate thatβ-cell extraction, combined with UPLC/MS, is a valid method for screening antidiabetic components from herbal medicines.

Print Options


Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.