Abstract Title:

The Effect of Silencing HIF-1α Gene in BxPC-3 Cell Line on Glycolysis-Related Gene Expression, Cell Growth, Invasion, and Apoptosis.

Abstract Source:

Nutr Cancer. 2015 Nov-Dec;67(8):1314-23. Epub 2015 Nov 17. PMID: 26576476

Abstract Author(s):

Yi Jiang, Guo-Hao Wu, Guo-Dong He, Qiu-Lin Zhuang, Qiu-Lei Xi, Bo Zhang, Yu-Song Han, Jing Fang

Article Affiliation:

Yi Jiang

Abstract:

Hypoxia has been proved to be a typical character of solid tumors. Tumor cells prefer to use glucose through the glycolysis pathway instead of aerobic respiration. However, the precise molecular mechanism underlying this so-called Warburg effect remains elusive. In the current study, siRNA was synthesized and transfected into BxPC-3 cell line to silence the expression of HIF-1α gene. It was found that hypoxia induced hypoxia-inducible factor 1α (HIF-1α) overexpression in BxPC-3 cells, enhanced the expression of pyruvate dehydrogenase kinase 1 and lactate dehydrogenase A, thus facilitating glycolysis and making tumor cells more tolerant to hypoxic stress. The silencingof HIF-1α gene significantly attenuated glycolysis under hypoxic conditions, inhibited the growth and invasion ability of BxPC-3 cells, and enhanced hypoxia-induced cell apoptosis.

Study Type : In Vitro Study
Additional Links
Additional Keywords : Warburg Effect : CK(22) : AC(18)

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