Abstract Title:

Bisphenol A and its analogues disrupt centrosome cycle and microtubule dynamics in prostate cancer.

Abstract Source:

Endocr Relat Cancer. 2016 Dec 20. Epub 2016 Dec 20. PMID: 27998958

Abstract Author(s):

Shuk-Mei Ho, Rahul C Rao, Sarah To, Emma Schoch, Pheruza Tarapore

Article Affiliation:

Shuk-Mei Ho


Humans are increasingly exposed to structural analogues of bisphenol A (BPA), as BPA is being replaced by these compounds in BPA-free consumer products. We have previously shown that chronic and developmental exposure to BPA is associated with increased prostate cancer (PCa) risk in human and animal models. Here we examine whether exposure of PCa cells (LNCaP, C4-2) to low-dose BPA and its structural analogues (BPS, BPF, BPAF, TBBPA, DMBPA and TMBPA) affects centrosome amplification (CA), a hallmark of cancer initiation and progression. We found that exposure to BPA, BPS, DMBPA and TBBPA in descending order, increased number of cells with CA, in a non-monotonic dose-response manner. Furthermore, cells treated with BPA and these analogues initiated centrosome duplication at 8h post-release from serum-starvation, significantly earlier in G-1 phase than control cells. This response was attended by earlier release of nucleophosmin from unduplicated centrosomes. BPA exposed cells exhibited increased expression of cyclin dependent kinase CDK6, and decreased expression of CDK inhibitors (p21Waf1/CIP1, p27KIP1). Using specific antagonists for estrogen/androgen receptors, CA in presence of BPA or its analogues was likely to be mediated via ESR1 signaling. Change in microtubule dynamics was observed on exposure to these analogues, which, for BPA, was accompanied by increased expression of centrosome-associated protein CEP350. Similar to BPA, chronic treatment of cells with DMBPA, but not other analogues, resulted in enhancement of anchorage-independent growth. We thus conclude that selected BPA analogues, similar to BPA, disrupt centrosome function and microtubule organization, with DMBPA displaying the broadest spectrum of cancer-promoting effects.

Study Type : In Vitro Study

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