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Abstract Title:

[Effects of Sinomenine on Proliferation and Apoptosis of MCL Jeko-1 Cell Line and Its Mechanism].

Abstract Source:

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Dec ;25(6):1675-1679. PMID: 29262896

Abstract Author(s):

Shu-Yu Chen, Yong Zou, Yi-Qun Huang

Article Affiliation:

Shu-Yu Chen

Abstract:

OBJECTIVE: To investigate the effects of sinomenine on growth and apoptosis of MCL Jeko-1 cell line and its mechanism.

METHODS: The proliferation rate of Jeko-1 cells treated by different doses of sinomenine was assayed by MTT method; and the cell apoptosis was detected by flow cytometry. The expressions of Cyclin D1, BCL-2, BAX, Caspase-3, Akt signaling pathway protein Akt, phosphorylated-Akt (p-Akt), and phosphorylated-mTOR (p-mTOR), phosphorylated- P70S6K(p-P70S6K) were determined by Western blot.

RESULTS: The growth of Jeko-1 cell line was inhibited by Sinomenine. The apoptosis rates of Jeko-1 cells treated by 0, 1, 2, and 4 mmol /L of Sinomenine for 24 hours were (2.21±1.05) %, (11. 29±2.42)%, (18.79±2.84) %, (31.05±3.52) % respectively, and with very statistically significant difference(P<0.01). The expressions of p-Akt, p-mTOR, p-P70S6K were down-regulated, but total Akt expression was not changed. The expressions of cyclin D1 and BCL-2 were down-regulated, but that of BAX, and Caspase-3 were up-regulated.

CONCLUSION: The sinomenine can inhibit Jeko-1 cell proliferation, which may be realized through down-regulating the phosphorylation level of p-Akt, p-mTOR, and p-P70S6K, thus inhibiting the Akt signaling pathway and promoting the cell apoptosis.

Study Type : In Vitro Study

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