n/a
Article Publish Status: FREE
Abstract Title:

β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model.

Abstract Source:

Nutrients. 2022 Jul 14 ;14(14). Epub 2022 Jul 14. PMID: 35889851

Abstract Author(s):

Young-Sool Hah, Won Keong Lee, Sangyeob Lee, Eun Ji Kim, Jung Hyeon Lee, Seung-Jun Lee, Yeong Ho Ji, Sang Gon Kim, Hyeong-Hwan Lee, Seo Yeon Hong, Jun-Il Yoo

Article Affiliation:

Young-Sool Hah

Abstract:

Sarcopenia refers to a decline in muscle mass and strength with age, causing significant impairment in the ability to carry out normal daily functions and increased risk of falls and fractures, eventually leading to loss of independence. Maintaining protein homeostasis is an important factor in preventing muscle loss, and the decrease in muscle mass is caused by an imbalance between anabolism and catabolism of muscle proteins. Althoughβ-sitosterol has various effects such as anti-inflammatory, protective effect against nonalcoholic fatty liver disease (NAFLD), antioxidant, and antidiabetic activity, the mechanism ofβ-sitosterol effect on the catabolic pathway was not well known.β-sitosterol was assessed in vitro and in vivo using a dexamethasone-induced muscle atrophy mice model and C2C12 myoblasts.β-sitosterol protected mice from dexamethasone-induced muscle mass loss. The thickness of gastrocnemius muscle myofibers was increased in dexamethasone with theβ-sitosterol treatment group (DS). Grip strength and creatine kinase (CK) activity were also recovered whenβ-sitosterol was treated. The muscle loss inhibitory efficacy ofβ-sitosterol in dexamethasone-induced muscle atrophy in C2C12 myotube was also verified in C2C12 myoblast.β-sitosterol also recovered the width of myotubes. The protein expression of muscle atrophy F-box (MAFbx) was increased in dexamethasone-treated animal models and C2C12 myoblast, but it was reduced whenβ-sitosterol was treated. MuRF1 also showed similar results to MAFbx in the mRNA level of C2C12 myotubes. In addition, in the gastrocnemius and tibialis anterior muscles of mouse models, Forkhead Box O1 (FoxO1) protein was increased in the dexamethasone-treated group (Dexa) compared with the control group and reduced in the DS group. Therefore,β-sitosterol would be a potential treatment agent for aging sarcopenia.

Study Type : Animal Study, In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.