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Article Publish Status: FREE
Abstract Title:

Polyphenols-Rich Fraction fromLinn. Leaves Attenuates Oxidative and Inflammatory Responses in Neutrophils, Macrophages, and Experimental Lung Injury.

Abstract Source:

Pharmaceutics. 2022 May 31 ;14(6). Epub 2022 May 31. PMID: 35745755

Abstract Author(s):

André Lopes Saraiva, Allisson Benatti Justino, Rodrigo Rodrigues Franco, Heitor Cappato Guerra Silva, Felipe Dos Santos Arruda, Sandra Gabriela Klein, Mara Rúbia Nunes Celes, Luiz Ricardo Goulart, Foued Salmen Espindola

Article Affiliation:

André Lopes Saraiva

Abstract:

Linn. is a common plant found in the warmest regions of South and Central America and its use in traditional medicine has been reported for the treatment of various illnesses. In the current study, we investigate the antioxidant and anti-inflammatory activities of crude extract and fractions fromL. leaves in isolated murine phagocytic immune cells as well as experimental LPS-induced acute lung injury (ALI). In a luminol-dependent chemiluminescence assay, we showed that ethyl acetate (EtOAc.f) and n-butanol (BuOH.f) fractions-both rich in polyphenols-reduced the generation of reactive oxygen species (ROS) by neutrophils stimulated with opsonized zymosan; similar results were found in culture of bone marrow-derived macrophages (BMDMs). By evaluating anti-inflammatory activity in BMDMs, EtOAc.f and BuOH.f reduced secretion of IL-6 and expression of the co-stimulatory molecule CD40. Furthermore, in LPS-induced ALI, oral administration of EtOAc.f reduced myeloperoxidase (MPO) activity in lung tissue. In addition, on a mechanism dependent on glutathione levels, the oxidative damage was also attenuated. These findings revealed direct antioxidant and anti-inflammatory activities of polyphenols-rich fractions ofL. leaves on neutrophils and macrophages. Moreover, the reduced oxidative damage and levels of inflammatory markers in experimental ALI suggest that these fractions might be explored for the development of new therapies for inflammatory conditions.

Study Type : In Vitro Study

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