Spinach contains an opioid peptide. - GreenMedInfo Summary

Abstract Title:

Rubiscolin, a delta selective opioid peptide derived from plant Rubisco.

Abstract Source:

FEBS Lett. 2001 Dec 7;509(2):213-7. PMID: 11741591

Abstract Author(s):

S Yang, J Yunden, S Sonoda, N Doyama, A W Lipkowski, Y Kawamura, M Yoshikawa

Article Affiliation:

Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.


We found that the sequences YPLDL and YPLDLF in the large subunit of spinach D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) met the structure YP-aliphatic amino acid which might have opioid activity. We then synthesized these peptides to test their opioid activity. The IC(50) of these peptides in mouse vas deferens assay were 51.0 microM and 24.4 microM, respectively, and those in delta receptor binding assay using [(3)H]deltorphin II as radioligand were 2.09 microM and 0.93 microM, respectively. Both peptides were selective for delta receptor. We named them rubiscolin-5 and -6, respectively. Rubiscolin-5 and -6 have antinociceptive activity in mice after i.c.v. or oral administration. The enzymatic conditions to release rubiscolin were investigated using both spinach Rubisco and synthetic fragment peptides. This is the first example of bioactive peptides derived from plant Rubisco.

Study Type : In Vitro Study
Additional Links
Pharmacological Actions : Analgesics : CK(5546) : AC(958)
Additional Keywords : Food Opioids : CK(20) : AC(14)

Print Options

Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.