Abstract Title:

Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay.

Abstract Source:

J Ethnopharmacol. 2012 Jun 14 ;141(3):825-30. Epub 2012 Mar 21. PMID: 22465512

Abstract Author(s):

Muhammad Zubair, Anders Ekholm, Hilde Nybom, Stefan Renvert, Cecilia Widen, Kimmo Rumpunen

Article Affiliation:

Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.

Abstract:

ETHNOPHARMACOLOGICAL RELEVANCE: The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago major L. (plantain) on cell proliferation and migration in vitro, as a test for potential wound healing properties.

MATERIALS AND METHODS: Water and ethanol-based extracts were prepared from Plantago major fresh and dried leaves, and tested in vitro in a scratch assay with oral epithelial cells.

RESULTS: The scratch assay produced reliable results after 18h. Most of the tested extracts increased the proliferation/migration of the oral epithelial cells compared to the negative control. A concentration of 1.0mg/mL (on dry weight basis) appears to be optimal regardless of type of extract, and among the alternatives, 0.1mg/mL was always better than 10mg/mL. Ethanol-based extracts with a concentration of 10mg/mL had very detrimental effects on cell proliferation/migration. At the other two concentrations, ethanol-based extracts had the most beneficial effect, followed by water extracts of fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves.

CONCLUSIONS: This study suggests that both the water extracts and the more polyphenol-rich ethanol-based extracts of Plantago major leaves have medicinal properties. Further research is, however, needed to determine what compounds are responsible for the wound healing effects.

Study Type : Human In Vitro
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