Abstract Title:

Effects of Plantago major L. leaf extracts on oral epithelial cells in a scratch assay.

Abstract Source:

J Ethnopharmacol. 2012 Jun 14 ;141(3):825-30. Epub 2012 Mar 21. PMID: 22465512

Abstract Author(s):

Muhammad Zubair, Anders Ekholm, Hilde Nybom, Stefan Renvert, Cecilia Widen, Kimmo Rumpunen

Article Affiliation:

Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Balsgård, Fjälkestadsvägen 459, 291 94 Kristianstad, Sweden.


ETHNOPHARMACOLOGICAL RELEVANCE: The present study was undertaken to evaluate the effects from different leaf extracts of the traditional medicinal herb Plantago major L. (plantain) on cell proliferation and migration in vitro, as a test for potential wound healing properties.

MATERIALS AND METHODS: Water and ethanol-based extracts were prepared from Plantago major fresh and dried leaves, and tested in vitro in a scratch assay with oral epithelial cells.

RESULTS: The scratch assay produced reliable results after 18h. Most of the tested extracts increased the proliferation/migration of the oral epithelial cells compared to the negative control. A concentration of 1.0mg/mL (on dry weight basis) appears to be optimal regardless of type of extract, and among the alternatives, 0.1mg/mL was always better than 10mg/mL. Ethanol-based extracts with a concentration of 10mg/mL had very detrimental effects on cell proliferation/migration. At the other two concentrations, ethanol-based extracts had the most beneficial effect, followed by water extracts of fresh leaves, ethanol plus water extracts of dried leaves and, finally, water extracts of dried leaves.

CONCLUSIONS: This study suggests that both the water extracts and the more polyphenol-rich ethanol-based extracts of Plantago major leaves have medicinal properties. Further research is, however, needed to determine what compounds are responsible for the wound healing effects.

Study Type : Human In Vitro
Additional Links

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.