Abstract Title:

In vitro antiproliferative effects of sulforaphane on human colon cancer cell line SW620.

Abstract Source:

Acta Medica (Hradec Kralove). 2007;50(3):171-6. PMID: 18254269

Abstract Author(s):

Hana Andelová, Emil Rudolf, Miroslav Cervinka

Article Affiliation:

Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Czech Republic. [email protected]


The isothiocyanate sulforaphane (SF) has been reported to possess chemopreventive efficiency towards various malignancies including colon cancer. Here, we investigated the antiproliferative and pro-apoptotic effects of SF on colon cancer cell line SW620. We found that SF at concentrations of 10-50 microM inhibits cell viability and proliferation of SW620 cells in a time- and dose-dependent manner, with IC50 being 26 microM (24 h), 24.4 microM (48 h) and 18 microM (72 h). Also, in the same cells SF caused DNA damage and chromatin condensation after 24 h and 48 h as revealed by phospho-H2A.X western blot analysis and DAPI staining of nuclei. These changes were accompanied by the elevated activity of caspase 3, although after 20 microM SF concentration only. Together, these results indicate that SF suppresses growth of human metastacic colonocytes and induces apoptotic cell death.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.