Abstract Title:

Effects of phytochemicals sulforaphane on uridine diphosphate-glucuronosyltransferase expression as well as cell-cycle arrest and apoptosis in human colon cancer Caco-2 cells.

Abstract Source:

Chin J Physiol. 2012 Apr 30 ;55(2):134-44. PMID: 22559738

Abstract Author(s):

Min Wang, Shuo Chen, Shuai Wang, Defeng Sun, Jian Chen, Yanqing Li, Wei Han, Xiaoyun Yang, Hai-Qing Gao

Article Affiliation:

Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University Key Laboratory of Proteomics of Shandong Province, Jinan Shandong, People's Republic of China. [email protected]


Our study investigated the effects of the chemopreventive agent sulforaphane (SFN) on human colon cancer Caco-2 cells and potential underlying mechanisms of the effects. When treated with SFN at hypotoxic levels, UDP-glucuronosyltransferase 1A (UGT1A) was induced in a dose-dependent manner. SFN at 25μM showed the highest UGT1A-induction activity, inducing UGT1A1, UGT1A8, and UGT1A10 mRNA expression, and increasing UGT-mediated N-hydroxy-PhIP glucuronidation. SFN- induced UGT1A expression may have resulted from Nrf2 nuclear translocation or activation. At higher concentrations, e.g. 75 μM, SFNcaused G1/G2 cell cycle arrest and induced apoptosis possibly through reducing anti-apoptotic bcl-2 expression and increasing apoptosis-inducing bax expression in Caco-2 cells. Taken together, these results demonstrated the chemopreventive effects of SFN on human colon cancer Caco-2 cells may havebeen partly attributed to Nrf2-mediated UGT1A induction and apoptosis induction, and our studies provided theoretic and experimental basis for clinical application of SFN to human colon cancer prevention.

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