Article Publish Status: FREE
Abstract Title:

The Synergistic Combination of Cisplatin and Piperine Induces Apoptosis in MCF-7 Cell Line.

Abstract Source:

Iran J Public Health. 2021 May ;50(5):1037-1047. PMID: 34183962

Abstract Author(s):

Abolfazl Fattah, Ali Morovati, Zahra Niknam, Ladan Mashouri, Amirhooman Asadi, Shirin Tvangar Rizi, Mojtaba Abbasi, Fatemeh Shakeri, Omid Abazari

Article Affiliation:

Abolfazl Fattah


Background: Piperine is a natural compound obtained from thethat exhibits anti-proliferative and anti-cancer activity in cancer cell lines. We analyzed the cytotoxic effect of piperine combined with cisplatin compound in the human MCF-7 breast cancer cell line and the underlying mechanism.

Methods: The present in vitro study was performed on MCF-7 cell line in Jahrom University of Medical Sciences between, Jahrom, Iran from 2016 to 2017. Cultured MCF-7 cells were seeded into four groups: a control group (untreated group), a group treated with cisplatin, a group treated with piperine and a group treated with cisplatin and piperine. Cell viability was analyzed using the MTT assay method. Flow c-ytometric analysis was investigated for apoptosis. The mRNA and protein expression of the apoptotic regulators p53, Bcl-2, Bax, caspase 3 and caspase 9 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting analysis.

Results: Piperine (20 and 30μM) in combination with cisplatin (5, 10 and 15 μM) for 24 h synergistically inhibited cell viability of MCF-7 breast cancer cells more than piperine and cisplatin used alone. Synergistic anti-breast cancer activities cisplatin (5 μM) and piperine (20 μM) were via inducing apoptosis. Piperine (20 μM) and cisplatin (5 μM) for 24 h induce apoptosis strongly through reduction of Bcl-2 and increase of caspase 3, p53, caspase 9, and Bax.

Conclusion: Piperine in combination with cisplatin could trigger p53-mediated apoptosis more effective than cisplatin alone in MCF-7 breast cancer cells, reducing the toxic dose of cisplatin used in cancer chemotherapy.

Study Type : In Vitro Study

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