n/a
Article Publish Status: FREE
Abstract Title:

Tanshinone IIA Accomplished Protection against Radiation-Induced Cardiomyocyte Injury by Regulating the p38/p53 Pathway.

Abstract Source:

Mediators Inflamm. 2022 ;2022:1478181. Epub 2022 Aug 22. PMID: 36046762

Abstract Author(s):

Gang Wang, Li Ma, Bowen Wang, Fentang Gao, Jianfeng Li, Hongyi Cai, Juan Wang, Tiancheng Zhang, Hao Guo, Ping Xie, Yi Li

Article Affiliation:

Gang Wang

Abstract:

Background: Radiotherapy is one of the major strategies for treating tumors, and it inevitably causes damage to relevant tissues and organs during treatment. Radiation-induced heart disease (RIHD) refers to radiation-induced cardiovascular adverse effects caused by thoracic radiotherapy. Currently, there is no uniform standard in the treatment of RIHD.

Methods: In our group study, by administering a dose of 4 Gy radiation, we established a radiation injured cardiomyocyte model and explored the regulatory relationship between tanshinone IIA and p38 MAPK in cardiomyocyte injury. We assessed cell damage and proliferation using clonogenic assay and lactate dehydrogenase (LDH) release assay. The measures of antioxidant activity and oxidative stress were conducted using superoxide dismutase (SOD) and reactive oxygen species (ROS). The apoptosis rate and the relative expression of apoptotic proteins were conducted using flow cytometry and western blot. To assess p38 and p53 expressions and phosphorylation levels, western blot was performed.

Results: Experimental results suggested that tanshinone IIA restored cell proliferation in radiation-induced cardiomyocyte injury (∗∗<0.01), and the level of LDH release decreased (∗<0.05). Meanwhile, tanshinone IIA could decrease the ROS generation induced by radiation (∗∗<0.01) and upregulate the SOD level (∗∗<0.01). Again, tanshinone IIA reduced radiation-induced cardiomyocyte apoptosis (∗∗<0.01). Finally, tanshinone IIA downregulated radiation-induced p38/p53 overexpression (∗∗∗<0.001).

Conclusions: The treatment effects of tanshinone IIA against radiation-induced myocardial injury may be through the regulation of the p38/p53 pathway.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.