Article Publish Status: FREE
Abstract Title:

Immunomodulatory Effects ofPolysaccharide Extract and Its Uptake Behaviors at the Cellular Level.

Abstract Source:

Molecules. 2020 Mar 16 ;25(6). Epub 2020 Mar 16. PMID: 32188121

Abstract Author(s):

Le Feng, Xiao Xiao, Jing Liu, Junyan Wang, Nan Zhang, Tao Bing, Xiangjun Liu, Ziping Zhang, Dihua Shangguan

Article Affiliation:

Le Feng


L. is a widely used functional food and medicinal herb in Asian countries.polysaccharides (LBP) are considered as one of the major medicinal components offruit and exhibits a wide range of biological activities. Here, we investigated the immunomodulatory effects of LBP and its uptake behaviors at the cellular level. LBP was prepared by water extraction and ethanol precipitation, and divided into two fractions based on the molecular weight distribution by ultrafiltration (LBP>10 kDa and LBP<10 kDa). The physicochemical properties of LBP and LBP fractions were well characterized. The LBP>10 kDa fraction greatly enhanced the viability of macrophages RAW264.7 cells and induced cell polarization, but had weak effects to other tested tumor cell lines and normal cell line. This fraction could regulate the production of NO, TNF-α, IL-6 and ROS in RAW264.7 cells, suggesting both pro-inflammatory and anti-inflammatory effects. The dye-labeled LBP could be internalized into all tested cell lines and accumulated in lysosomes. The internalization of LBP in RAW264.7 cells is mainly through the clathrin-mediated endocytosis pathway. The Caco-2 intestinal transport experiment demonstrated that the dye labeled LBP could be transported through the Caco-2 cell monolayer (mimic intestinal epithelium) through clathrin-mediated endocytosis. These results demonstrate the immunomodulatory effects of LBP and its effective uptake bymacrophages and intestine.

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