Article Publish Status: FREE
Abstract Title:

The effects of methanolic, chloroform, and ethylacetate extracts of the Cucurbita pepo L. on the delay type hypersensitivity and antibody production.

Abstract Source:

Res Pharm Sci. 2012 Oct ;7(4):217-24. PMID: 23248672

Abstract Author(s):

A Jafarian, B Zolfaghari, M Parnianifard

Article Affiliation:

A Jafarian


Pumpkin, as a dietary plant, has been used in traditional medicine around the world. In addition, during the last decade, antidiabetic, antihypertensive, antitumor, intestinal antiparasitic, antibacterial, anti hypercholesterolemia, anti-inflammatory, immunomodulatory and analgic effects of pumpkin has been reported. The aim of the present study was to determine the effects of different extracts of Cucurbita pepo L. on the immune responses. Methanolic, chloroform and ethylacetate extracts of C. pepo fruits was obtained using percolation method. Mice were used to study the effects of C. pepo extracts on the acquired immunity. Sheep red blood cell (SRBC) was injected (S.C., 1×10(8) cells/ml, 20 μl) and 5 days later, methanolic, chloroform and ethylacetate extracts of C. pepo at diiferent doses (10, 100 and 500 mg/kg), betamethasone and levamisol at equal doses (4 mg/kg) as positive controls and normal saline as a negative control were given i.p. After 1 h SRBC was injected to the footpad (S.C., 1×10(8) cells/ml, 20 ml) and the footpad swelling was measured up to 72 h. To investigate the effects of C. pepo on the innate immunity the same procedure was used, but animals received only one injection of SRBC 1 h after i.p. injection of test compounds. Our findings showed that SRBC induced an increase in the paw swelling with maximum response at 6-8 h. Betamethasone inhibited the paw swelling in both models. In both innate and acquired immunity models, methanolic, chloroform and ethylacetate extracts of C. pepo fruits significantly reduced the paw swelling dosedependently. The data suggest that the pumpkin extracts may have immunomodulatory effects.

Study Type : In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.