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Article Publish Status: FREE
Abstract Title:

Ursolic Acid Targets Glucosyltransferase and Inhibits Its Activity to PreventBiofilm Formation.

Abstract Source:

Front Microbiol. 2021 ;12:743305. Epub 2021 Sep 27. PMID: 34646258

Abstract Author(s):

Yucui Liu, Yanxin Huang, Cong Fan, Zhongmei Chi, Miao Bai, Luguo Sun, Li Yang, Chunlei Yu, Zhenbo Song, Xiaoguang Yang, Jingwen Yi, Shuyue Wang, Lei Liu, Guannan Wang, Lihua Zheng

Article Affiliation:

Yucui Liu

Abstract:

, the prime pathogen of dental caries, can secrete glucosyltransferases (GTFs) to synthesize extracellular polysaccharides (EPSs), which are the virulence determinants of cariogenic biofilms. Ursolic acid, a type of pentacyclic triterpene natural compound, has shown potential antibiofilm effects on. To investigate the mechanisms of ursolic acid-mediated inhibition ofbiofilm formation, we first demonstrated that ursolic acid could decrease the viability and structural integrity of biofilms, as evidenced by XTT, crystal violet, and live/dead staining assays. Then, we further revealed that ursolic acid could compete with the inherent substrate to occupy the catalytic center of GTFs to inhibit EPS formation, and this was confirmed by GTF activity assays, computer simulations, site-directed mutagenesis, and capillary electrophoresis (CE). In conclusion, ursolic acid can decrease bacterial viability and preventbiofilm formation by binding and inhibiting the activity of GTFs.

Study Type : In Vitro Study

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