Abstract Title:

Vitamin C Prevents Offspring DNA Methylation Changes Associated with Maternal Smoking in Pregnancy.

Abstract Source:

Am J Respir Crit Care Med. 2017 Apr 19. Epub 2017 Apr 19. PMID: 28422514

Abstract Author(s):

Lyndsey E Shorey-Kendrick, Cindy T McEvoy, Betsy Ferguson, Julja Burchard, Byung S Park, Lina Gao, Brittany H Vuylsteke, Kristin F Milner, Cynthia D Morris, Eliot R Spindel

Article Affiliation:

Lyndsey E Shorey-Kendrick


BACKGROUND: Infants whose mothers smoked during pregnancy demonstrate lifelong decreases in pulmonary function. DNA methylation changes associated with maternal smoking during pregnancy have been described in placenta and cord blood at delivery, in fetal lung, and in buccal epithelium and blood during childhood. We recently demonstrated in a randomized clinical trial (NCT00632476) that vitamin C supplementation to pregnant smokers can lessen the impact of maternal smoking on offspring pulmonary function and decrease the incidence of wheeze at one year of age.

OBJECTIVES: To determine if vitamin C supplementation reduces changes in offspring methylation in response to maternal smoking and whether methylation at specific CpGs is also associated with respiratory outcomes.

METHODS: Targeted bisulfite sequencing was performed using a subset of placentas, cord bloods and buccal samples collected during the NCT00632476 trial followed by independent validation of selected cord blood DMRs using bisulfite amplicon sequencing.

RESULTS: The majority (69.03%) of CpGs with≥ 10% methylation difference between placebo and non-smoker groups were restored (by at least 50%) towards non-smoker levels with vitamin C treatment. A significant proportion of restored CpGs were associated with phenotypic outcome with greater enrichment among hypomethylated CpGs.

CONCLUSIONS: We identified a pattern of normalization in DNA methylation by vitamin C supplementation across multiple loci. The consistency of this pattern across tissues and time suggests a systemic and persistent effect on offspring DNA methylation. Further work is necessary to determine how genome-wide changes in DNA methylation may mediate or reflect persistent effects of maternal smoking on lung function.

Study Type : Human Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.