Vitexin alleviates interleukin-1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF-1α pathway.
Scand J Immunol. 2019 May 4:e12773. Epub 2019 May 4. PMID: 31055848
It has been reported that vitexin has anti-inflammatory effects in osteoarthritis (OA) rats. However, the effects of vitexin on interleukins 1β (IL-1β) stimulated OA patient-derived chondrocytes have not been reported. The purpose of this study was to investigate the anti-inflammatory effects of vitexin on IL-1β stimulated human osteoarthritis chondrocytes and to reveal the involvement of hypoxia-inducible-factor 1α (HIF-1α) pathway.Enzyme-linked immunosorbent assay, quantitative real-time PCR and Western blotting assays were employed. ELISA results demonstrated that the proinflammatory cytokine levels of interleukins 6 (IL-6) and tumor necrosis factor α (TNF-α) in the serum and synovial fluid and HIF-1α level in the synovial fluid were significantly elevated in OA patients compared to normal healthy subjects. Moreover, the Western blotting results indicated that the protein expression of HIF-1α was significantly higher in the cartilage tissues of OA patients. OA patient-derived chondrocytes were stimulated by IL-1βand treated with different concentration of vitexin for 24 h. Vitexin showed no cytotoxicity and increased the survival of chondrocytes under IL-1β stimulation. Vitexin suppressed IL-1β induced production of NO and Prostaglandin E2 (PGE) in chondrocytes culture. The treatment of vitexin significantly inhibited IL-1β induced expressions of proinflammatory cytokine levels of IL-6, TNF-α, matrix metalloproteinase (MMP) 1, MMP-3 and MMP-13. Furthermore, Western blotting results demonstrated that HIF-1α is involved in vitexin's protective effects on IL-1β stimulated injuries in OA patient-derived chondrocytes.Our study demonstrate that vitexin alleviates IL-1β induced inflammatory responses in chondrocytes from osteoarthritis patients, which may be attributed partly to the inhibition of HIF-1α pathway. This article is protected by copyright. All rights reserved.