In vitro anti-biofilm activities of citral and thymol against candida tropicalis. - GreenMedInfo Summary
In Vitro Anti-Biofilm Activities of Citral and Thymol Against Candida Tropicalis.
J Fungi (Basel). 2019 Feb 3 ;5(1). Epub 2019 Feb 3. PMID: 30717454
is an emerging non-species which is pathogenic to the immune-compromised humans, especially in tropical countries, including India. The acquired resistance ofspecies towards antifungal therapies is of major concern. Moreover, limited efficacy and dosage constraint of synthetic drugs have indicated the prerequisite of finding new and natural drugs for treatment. In the present study, we have compared the influence of citral and thymol onand its biofilm along with expression levels of certain antifungal tolerance genes. The antifungal and anti-biofilm activities of the both were studied using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT) reduction assay, field emission scanning electron microscope (FE-SEM) and confocal laser scanning microscope (CLSM) and real-time reverse transcription polymerase chain reaction (RT-PCR) analysis. Citral and thymol have damaged the cells with distorted surface and less viability. Quantitative real-time PCR analysis showed augmented expression of the cell membrane biosynthesis genes including/against citral and the cell wall related tolerance genes involvingagainst thymol thus, depicting their differential mode of actions.