In vitro antifungal activity of olive leaf extracts loaded in chitosan nanoparticles. - GreenMedInfo Summary
Antifungal Activity of Olive () Leaf Extracts Loaded in Chitosan Nanoparticles.
Front Bioeng Biotechnol. 2020 ;8:151. Epub 2020 Mar 3. PMID: 32195234
Olive leaf extract is characterized by a high content of phenols and flavonoids (oleuropein, luteolin, and their derivatives). These compounds are defined as secondary metabolites and exert such as anti-inflammatory, antioxidant, and antimicrobial activities. We investigated theantifungal activity of two olive leaf extracts (namedand) against a(AACC0215) strain that causes diseases to many economically important plants and synthesizing diverse mycotoxins. In this work, we aimed to identify the most appropriate concentration between the tested two olive leaf extracts to develop a safe, stable and efficient drug delivery system. Qualitative and quantitative analyses of the two olive leaf extracts by (HPLC) were performed. Furthermore, we also evaluated the antifungal effects of the two leaf extracts when encapsulated in chitosan-tripolyphosphate nanoparticles. The major compound in bothandwas oleuropein, with 336 and 603 mg/g, respectively, however, high concentrations of flavonoid were also present.andshowed a concentration depended effect on(AACC0215) viability. Our results showed a great efficacy of/nanoparticles at the higher concentration tested (12X) against the target species. In this case, we observed an inhibition rate to both germination and growth of 87.96 and 58.13%, respectively. We suggest thatolive leaf extracts, as free or encapsulated in chitosan-tripolyphosphate nanoparticles, could be used as fungicides to control plant diseases. Finally, future application of these findings may allow to reduce the dosage of fungicides potentially harmful to human health.