In Vitro Antileishmanial Effects of Saffron Compounds, Crocin and Stigmasterol, on Iranian Strain of(MHOM/IR/75/ER).
Iran J Parasitol. 2021 Jan-Mar;16(1):151-158. PMID: 33786057
Background: Due to numerous side effects of common drugs in treatment of leishmaniasis, new therapeutic approaches focus on herbal compounds. Therefore, we aimed to determine the effect of crocin and stigmasterol on in-vitro growth of promastigotes and amastigotes ofin the Department of Parasitology, Pasteur Institute, Tehran, Iran in 2018.
Methods: The effect of different concentrations of crocin and stigmasterol were evaluated by determining their in-vitro inhibitory effects on promastigotes and amastigotes of theusing MTT assay.
Results: The fatality rate was 65.27% and 71.96% for crocin and stigmasterol respectively at 24 h post-culture in concentration of 50μg/mL. The mean inhibitory effect of crocin and stigmasterol onamastigotes after 72 h were 52.22% and 38.96%.
Conclusion: The crocin and stigmasterol had efficient adverse effects on promastigote and amastigotes of, hence, further studies on the anti-leishmanial effects of these herbal compounds in human and animal models are recommended.