In Vitro Antiproliferative and Antioxidant Effects of Extracts fromLeaves and Their Quality Evaluation.
Evid Based Complement Alternat Med. 2016 ;2016:5698685. Epub 2016 Dec 22. PMID: 28101119
Daniel Mirosław Grochowski
The present study was performed to evaluate the effect of different extracts and subfractions fromleaves on two human colon cancer cell lines obtained from two stages of the disease progression lines HT29 and SW948. Tested samples inhibited the viability of cells, both HT29 and SW948 lines, in a concentration-dependent manner. The most active was the ethyl acetate fraction which, applied at the highest concentration (250 g/mL), decreased the viability of cells (HT29 and SW948) below 66%. The extracts and subfractions were also investigated for antioxidant activities on DPPH and FRAP assays. All extracts, with the exception of water extract at a dose of 250 g/mL, almost totally reduced DPPH. The highest Feion reduction was shown for the diethyl and ethyl acetate fractions. It was more than 6.5 times higher (at a dose 250 g/mL) as compared to the control. The LC-MS studies of the analysed preparations showed that all samples contain a wide variety of polyphenolics, among which ellagitannins turned out to be the main constituents with dominant ellagic acid, sanguiin H-6, and flavonol derivatives.