Protective Effect of Water Extractedon Glutamate-induced Neuronal Cell Death in Mouse Hippocampal HT22 Cell.
Pharmacogn Mag. 2018 Apr-Jun;14(54):242-247. Epub 2018 Apr 10. PMID: 29720839
Hyeon Yong Lee
Introduction: was used as important nutritional source in the Aztec civilization because it is rich in proteins and vitamins. It contains various antioxidants such as phycocyanin and flavonoids. Based on abundant antioxidants,is known to possess anti-inflammatory effect, especially on neuronal cells.
Materials and Methods: was extracted in water and contain of phycocyanin was identified by high-performance liquid chromatography. Cell viability test was performed with treatment ofextract. After, oxidative stress-related mechanisms were evaluated by detecting the accumulation of reactive oxygen species (ROS) and Cainflux, and decrease of mitochondrial membrane potential (MMP) level. Then, the glutathione (GSH) related assays were conducted.
Results: The water extractedexerted the neuroprotective activity by attenuating the ROS and Caformation, maintaining the MMP level, and protecting the activity of the antioxidant enzymes by increasing reduced GSH against oxidative stress compared to control.
Conclusion: The results suggested that water extractedshowed powerful neuroprotective effect through the mechanism related to antioxidant activity, able to preventing the radical-mediated cell death.
SUMMARY: Water extractedcontains C-phycocyaninWater extractedexerts neuroprotective effect on HT22 cellTo investigate the protective mechanisms, reactive oxygen species, Ca, mitochondrial membrane potential, Glutathione-related assays were performed.ROS: Reactive oxygen species; MMP: Mitochondrial membrane potential; GSH: Glutathione; GSSG: Glutathione disulfide, oxidized glutathione; GPx: Glutathione peroxidase; GR: Glutathione reductase; DMEM: Dulbecco's modified Eagle's medium; FBS: Fetal bovine serum; DCF-DA: 2',7'-dichlorofluorescein diacetate; PBS: Phosphate buffered serum; Rho 123: Rhodamine 123; NADPH: Nicotinamide adenine dinucleotide phosphate; DTNB: 5,5'-dithiobis-2-nitrobenzoic acid, Ellman's reagent; GSSG-R: Glutathione disulfide reductase; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; HPLC: High-performance liquid chromatography.