Abstract Title:

Withanolides from Withania somnifera Ameliorate Neutrophil Infiltration in Endotoxin-Induced Peritonitis by Regulating Oxidative Stress and Inflammatory Cytokines.

Abstract Source:

Planta Med. 2021 Apr 16. Epub 2021 Apr 16. PMID: 33862643

Abstract Author(s):

Acharya Balkrishna, Siva Kumar Solleti, Hoshiyar Singh, Niti Sharma, Anurag Varshney

Article Affiliation:

Acharya Balkrishna


Identification of novel anti-inflammatory strategies are needed to avoid the side effects associated with the currently available therapies. Use of anti-inflammatory herbal remedies is gaining attention. The purpose of the present investigation was to evaluate the pharmacological potential of the withanolide-rich root extracts of the medical plant(L.) Dunal usingandmodels of endotoxin-induced inflammation and oxidative stress. The pharmacological effects ofroot extracts were evaluated using a mouse model of endotoxin (lipopolysaccharide)-induced peritonitis and various relevant human cell lines. HPLC analysis of theroot extracts identified the presence of various bioactive withanolides.challenge of mice with endotoxin resulted in the infiltration of various leukocytes, specifically neutrophils, along with monocytes and lymphocytes into the peritoneal cavity. Importantly, prophylactic treatment withinhibited the migration of neutrophils, lymphocytes, and monocytes and decreased the release of interleukin-1, TNF-, and interleukin-6 cytokines into the peritoneal cavity as identified by ELISA. Liver (glutathione peroxidase, glutathione, glutathione disulfide,, m, myeloperoxidase) and peritoneal fluid (nitrite) biochemical analysis revealed the antioxidant profile of. Similarly, in human HepG2 cells,significantly modulated the antioxidant levels. In THP-1 cells,decreased the secretion of interleukin-6 and TNF-. In HEK-Blue reporter cells,inhibited TNF--induced nuclear factor-B/activator protein 1 transcriptional activity. Our findings suggest the pharmacological effects of root extracts ofrich in withanolides inhibit neutrophil infiltration, oxidative hepatic damage, and cytokine secretion via modulating the nuclear factor-B/activator protein 1 pathway.

Print Options

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.