n/a
Abstract Title:

[Effects of Wogonin on Apoptosis,Invasion,Migration and Wnt/β-Catenin Signaling Pathway of Gastric Cancer Cells SGC7901].

Abstract Source:

Zhong Yao Cai. 2016 Jun ;39(6):1372-6. PMID: 30156810

Abstract Author(s):

Xiao-hui Wang, Ying-fang Wei, Hong Cheng

Article Affiliation:

Xiao-hui Wang

Abstract:

Objective: To explore the effects of wogonin on the apoptosis,invasion,migration and Wnt /β-catenin signaling pathway of gastric cancer cells SGC7901.

Methods: Three common gastric cancer cell lines( SGC7901,BGC-823 and MKN-45) were conventionally cultured to logarithmic growth. The cell proliferation was detected by MTT assay after treatment with different concentrations of wogonin( 0,20,50,100 and 200μmol / L) for 24,48,72 and 96 h. The apoptosis rate, migration and invasion ability of SGC7901 cells were detected by Annexin V-FITC / PI annexin double staining flow cytometry, scratch test and transwell cell invasion assay, respectively. After treament with different concentrations of wogonin for48 h,the protein levels of β-catenin, C-myc and Cyclin D1 were detected by Western blotting.

Results: Wogonin within the range of 20 ~ 200 mol / L could inhibit the proliferation of SGC7901,BGC-823 and MKN-45 cells in a dose-and time-dependent manner. Compared with 0μmol / L wogonin group,After treament with different concentrations of wogonin for 24 h and 48 h,SGC7901 cells had elevated apoptosis rate and decreased migration distance and number of penetrating membrane cell in the rest concentrations( P<0. 05). The protein levels ofβ-catenin,C-myc and Cyclin D1 were lower in 20 ~ 200μmol /L wogonin groups than 0 μmol /L wogonin group except for the concentration of 20 μmol /L wogonin groups( P<0. 05),and the effects in a dose-dependent manner.

Conclusion: Wogonin has the ability to inhibit the proliferation of gastric cancer cells,and can induce apoptosis and inhibit cell migration and invasion,which may be related to the inhibition of Wnt /β-catenin signaling pathway activation.

Study Type : In Vitro Study

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.